In Situ Detection Method for Glutaminyl Cyclase Activity in Polyacrylamide Gels
نویسندگان
چکیده
منابع مشابه
In situ detection method for glutaminyl cyclase activity in polyacrylamide gels.
that achieved by TLC with numerous buffers (1,2,5) or by HV-TLE protocols (2) when multiple samples are analyzed. We have found that when 1 μL of a dye solution (1% orange G, 1% phenol red in pH 3.5 buffer) was electrophoresed in parallel with the phosphoamino acid standards, orange G migrated slightly ahead of pSer and phenol red migrated with pTyr, providing useful visible markers for electro...
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METHOD 1. Immerse the gel, together with its attached glass plate, in 7% acetic acid for 5 minutes. Remove the gel from the fixative by carefully lifting the glass plate from the fluid. 2. Rinse the gel briefly in deionized H2O. Remove excess fluid from the surface of the gel with a pad of Kimwipes. IMPORTANT Do not use absorbent paper; it will stick to the gel. 3. (Optional) Dry the gel onto a...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98243bm02